Research using embryonic stem (ES) cells continues to increase, and serum suitability is critical for keeping these cells in their valuable undifferentiated state. Historically, researchers were required to screen several lots of serum to find one suitable for ES research. HyClone’s ES screened FBS eliminates the need for prescreening serum, saving both time and money, while providing the assurance that the serum used has been screened by experienced ES cell culturists. The screening includes plating efficiency, colony morphology, and toxicity tests. This protocol was adapted from Gene Targeting, A.L. Joyner, 1995, page 43. ES screened FBS is selected from our highest quality Defined FBS lots, and it is filtered through sequential 40nm (0.04 µm) pore-size rated filters. Further heat inactivation or irradiation processing can be done upon request.

Assays (subject to change without notice): Progesterone, Selenium, Insulin, IgG, Sodium, Cholesterol, Potassium, High Density Lipo-Protein Cholesterol, Calcium, Low Density Lipo-Protein Cholesterol, Chloride, Triglycerides, Magnesium, Gamma Globulin, Glucose, Phospholipids, Copper, Alkaline Phosphatase, Total Iron Binding Capacity (TIBC), Lactate Dehydrogenase, Percent Saturation, Glutamic Pyruvic Transaminase (SGPT), Glutamic Oxaloacetic Transaminase (SGOT), Thiamine (B1), Riboflavin (B2), Pyridoxine (B6), Nicotinic Acid (Niacin), Vitamin H (Biotin), Vitamin A (Retinol), Carotene, Total Vitamin E (Tocopherol), Cyanocobalamine (B12), Pteroylglutamic Acid (Folate), Vitamin C, 1,25 Dihydroxy Vitamin D, pH, Total Protein, Albumin, Blood Urea Nitrogen, Creatinine, Total Bilirubin, Inorganic Phosphorus, Osmolality, and Iron.