Dialyzed FBS is used in applications requiring serum depleted of small molecules (less than 10,000 mw) and is filtered through three sequential 100nm (0.1 µm) pore-size rated filters. HyClone has developed a diafiltration process that reduces concentrations of low molecular weight components such as nucleotides and amino acids that are necessary for alternative biochemical survival path ways. The process is reproducible and reduces hypoxanthine and thymidine concentrations below detectable limits. While producing the same results, a major difference between diafiltration and dialysis is that the driving force for diafiltration is hydrostatic pressure, rather than concentration gradients with dialysis. Monitoring glucose concentrations carefully controls the diafiltration process. The glucose concentration, which is representative of the extent of dialysis, is reported for each lot of serum.

Assays (subject to change without notice): Progesterone, Magnesium, Insulin, Selenium, Parathyroid Hormone (PTH), IgG, Cholesterol, Potassium, High Density Lipo-Protein Cholesterol, Calcium, Low Density Lipo-Protein Cholesterol Chloride, Triglycerides, Copper, Gamma Globulin, Glucose, Phospholipids, Osmolality, Alkaline Phosphatase, Iron, Lactate Dehydrogenase, Total Iron Binding Capacity (TIBC), Glutamic Pyruvic Transaminase (SGPT), Percent Saturation, Glutamic Oxaloacetic Transaminase (SGOT), Thiamine (B1), Riboflavin (B2), Pyridoxine (B6), Nicotinic Acid (Niacin), Vitamin H (Biotin), Vitamin A (Retinol), Carotene, Total Vitamin E (Tocopherol), Cyanocobalamine (B12), Pteroylglutamic Acid (Folate), Vitamin C, 1,25 Dihydroxy Vitamin D, pH, Total Protein, Albumin, Blood Urea Nitrogen, Creatinine, Total Bilirubin, Sodium, and Inorganic Phosphorus.