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Table 4.

Acid Treated FBS     11112568 A 2568   11112594 A 2594   11112620 A 2620 Test Units Original Acid   Original Acid   Original Acid   Endotoxin EU/ml <0.06 0.125   <0.06 <0.06   <0.06 <0.06 Hemoglobin mg/dl 7 4   7 3   10 6 Progesterone ng/dl <10 <10   4.1 <10   <10 <10 Insulin µU/ml 6.6 7.4   9.3 6   8.5 6 PTH pg/ml 46 39.8   33 60   149 40 Vitamin D 1,25 pg/ml 64 36   44 28   * 55 pH   7.51 7.53   7.46 7.48   7.47 7.44 Osmolality mosm/kg 304 353   299 349   309 357 Thiamine B1 ng/ml 172 131   95 125   93 125 Riboflavin B2 ng/ml 215 161   227 180   223 174 Pyridoxine B6 ng/ml 95 73   81 61   66 75 Niacin ng/ml 1.6 1   0.92 1   1.1 1 Biotin Vitamin H pg/ml 37,083 21,111   43,542 21,444   33,611 21,444 Retinol ng/ml 170 <100   130 <100   190 <100 Beta Carotene µg/ml 0.11 0.16   <0.1 0.16   0.16 0.16 Total Vitamin E µg/ml <1.0 <1.0   2 <1.0   <1.0 <1.0 Cobalamine B12 pg/ml 356 304   231 250   379 292 Folic Acid mg/ml 36 22   44 31   79 44 Vitamin C mg/dl <0.05 <0.05   0.8 <0.05   0.8 <0.05 Gamma Globulin %tp 0.5 <0.1   <0.1 <0.1   <0.5 <0.1 Total Protein gm% 3.3 3.4   3.4 3.7   3.7 3.8 Albumin gm% 2.4 2.4   2.6 2.7   2.8 2.7 Blood Urea Nitrogen md/dl 16 16   15 16   17 17 Uric Acid mg/dl 1.8 1.7   3.4 2.2   2.7 2.4 Creatine mg/dl 2.2 2.2   2.7 2.7   3 2.9 Total Bilirubin mg/dl 0.3 0.3   0.3 0.3   0.3 0.4 Sodium meq/L 136 164   136 163   139 165 Potassium meq/L 10.4 10.4   10 10.1   10.5 10.3 Calcium mg/dl 13.8 13.2   13.6 13.2   14.8 13.6 Chloride meq/dl 97 133   99 131   101 135 Inorganic Phosphorus mg/dl 10.2 10.8   9.3 10.4   10.7 10.7 Glucose mg/dl 86 78   89 85   112 105 Alkaline Phosphatase IU/L 206 140   259 193   246 187 Lactate Dehydrogenase IU/L 590 138   709 172   771 190 SGPT IU/L 5 6   8 5   7 4 SGOT IU/L 9 149   90 53   114 69 Cholesterol mg/dl 35 38   32 34   33 34 HDL mg/dl 9 12   11 13   10 13 LDL mg/dl 14 13   10 10   12 10 Triglycerides mg/dl 62 64   54 56   54 57 Phospholipids mg/dl 42 49   44 43   40 46 Copper µg/dl 0.14 0.1   0.13 0.12   0.16 0.18 Magnesium µg/ml 25.3 23.8   24.1 22.8   23.8 27.3 Selenium ng/ml 9 12   28 16   17 23 Iron µg/dl 160 126   176 117   173 138 TIBC µg/dl 242 214   226 181   317 218 Percent Saturation % 66 59   78 65   55 63 IgG mg/ml 0.108 0.1   0.132 0.11   0.22 0.2                     FOX-NY Hybrid % 96 102   90 112   114 104 MRC-5 % 101 114   104 100   101 98 CHO-K1 % 95 73   113 87   N/A 88 VERO % N/A 90   N/A 103   N/A 92 Blue Tongue Virus Titer TCID50/ml 6 <0.50   6.33 <0.50   6 <0.50   Keys to Our Success Section Navigation    Processes   Heat Inactivation - Are You Wasting Your Time?   Viral Safety in Serum for Cell Culture Use      Introduction   Assessment of Risk for use of Animal–Derived Material    Figure 1. Preliminary Raw Materials Risk Assessment Worksheet   Figure 2. Recipient Preliminary Risk Assessment   Regulatory Control   Gamma Irradiation - Serum    Table 1. Gamma Irradiation - Virus Recovery Matrix   Table 2. Irradiated FBS Analysis   Heat Treatment    Table 3. Heat Treatment Chart   Acid Treatment > Table 4. Acid Treatment Chart Table 5. Acid Treatment - Equine Serum Chart Total Sterility Assurance Article References