HyClone Laboratories, Inc.
 

 

FetalClone® II

FetalClone® II, US Origin
SH30066.02 100 mL
SH30066.03 500 mL

FetalClone® II, Heat Inactivated
SH30066.02HI 100 mL
SH30066.03HI 500 mL
FetalClone® II, Irradiated
SH30066.03IR 500 mL

FetalClone II is optimized for the growth of CHO cells and derivatives, offering the same basic formulation as FetalClone I, plus additional growth factors and supplements. No adaptation is required and suggested concentrations are the same as those used with FBS. This product has been successfully used with the cell lines shown in the table below.

Assays (subject to change without notice): Gamma Globulin, Alkaline Phosphatase, Lactate Dehydrogenase, Glutamic Pyruvic Transaminase (SGPT), Glutamic Oxaloacetic Transaminase (SGOT), pH, Total Protein, Albumin, Blood Urea Nitrogen, Creatinine, Total Bilirubin, Sodium, Potassium, Calcium, Chloride, Inorganic Phosphorus, Osmolality, Iron, Total Iron Binding Capacity (TIBC), Percent Iron Saturation, Glucose, and IgG.

Tests for SH30066 Specification
Endotoxin (Limulus Amebocyte Lysate - Gel Clot) <10 EU/mL
Hemoglobin (spectophotometric) <20 mg/dl
Sterility Testing (Current USP & EP)
Bacteria & Fungi No Growth
Virus Testing (9 CFR 113.53)
Flourescent Antibody
Bluetongue Not Detected
Bovine Adenovirus Not Detected
Bovine Parvovirus Not Detected
Bovine Respiratory Syncytial Virus Not Detected
Bovine Viral Diarrhea Virus Not Detected
Rabies Not Detected
Reovirus Not Detected
Cytopathogenic Agents - e.g IBR Not Detected
Hemadsorbing Agents - e.g. PI3 Not Detected
Mycoplasma
Large Volume, Direct Culture Not Detected
Hoechst DNA Stain Not Detected
Certificate of Suitability Included
Cell Lines grown successfully in Fetal Clone II
Cell Line Description
9HTEo Human epithelial trachea
BHK Baby hamster kidney fibroblast
C3H/10T1/2 Murine embryo fibroblast
CHO-CCKR Chinese hamster ovary
CHO-K1 Chinese hamster ovary
COS African Green monkey kidney fibroblast
Dg44/CHO-K1 Chinese hamster ovary
FOX-NY Murine myeloma
FOX-NY Hybrid Murine hybridoma
HEK-293 Human embryonic kidney
HeLa Human epithelial adenocarcinoma
Hep 3B2 Human liver epithelial carcinoma
HEp-2 Human epithelial adenosarcoma
JEG3 Human choriocarcinoma
Jurkat T-lymphocyte Human thymoma
L(-tk) Murine L cell connective tissue
L929 Murine areolar and adipose fibrosarcoma
MDBK Bovine kidney
NIH/3T3 Murine embryo fibroblast
NS-1 Murine myeloma
NS-1 Hybrid Murine hybridoma
P3 Murine myeloma
P3 Hybrid Murine hybridoma
P3X63 Ag8.653 Murine myeloma
P3X63 Ag8.653 Hybrid Murine hybridoma
Pancratic cells Murine
PC-12 Murine adrenal gland pheochromocytoma
SH-SY5Y Human neuroblastoma
SP2/0-Ag14 Murine myeloma
SP2/0-Ag14 Hybrid Murine hybridoma
U-937 Human histiocytic lymphoma

Products Section Navigation
Fetal Bovine Serum
Specialty FBS
Bovine Calf Serum
Fetal Bovine Replacement
  - Bovine Growth Serum(BGS)
  - FetalClone I
  - FetalClone II
  - FetalClone III
  - Alpha Calf Fraction
  - Alpha Calf Fraction Iron Supplemented
  - FetalClone III EMEA Conforming
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