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To provide hybridoma users with a cost-effective medium supplement that promotes growth and
antibody production, HyClone developed Alpha Calf Fraction. This product
is produced from our bovine calf serum using a nonethanol proprietary
process to reduce immunoglobulins and protein to levels very similar to
those of FBS. Alpha Calf Fraction typically contains less than 200µg/ml IgG.
Assays (subject to change without notice): Gamma Globulin, Alkaline Phosphatase, Lactate
Dehydrogenase, Glutamic Pyruvic Transaminase (SGPT), Glutamic Oxaloacetic
Transaminase (SGOT), pH, Albumin, Blood Urea Nitrogen, Creatinine, Total
Bilirubin, Sodium, Potassium, Calcium, Chloride, Inorganic Phosphorus,
Iron, Total Iron Binding Capacity (TIBC), Percent Saturation, and Glucose.
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| Endotoxin (Limulus Amebocyte Lysate - Gel Clot) |
<10 EU/mL |
| Hemoglobin (spectophotometric) |
<20 mg/dl |
| Sterility Testing (Current USP & EP) |
|
| Bacteria & Fungi |
No Growth |
| Virus Testing (9 CFR 113.53) |
|
| Flourescent Antibody |
|
| Bluetongue |
Not Detected |
| Bovine Adenovirus |
Not Detected |
| Bovine Parvovirus |
Not Detected |
| Bovine Respiratory Syncytial Virus |
Not Detected |
| Bovine Viral Diarrhea Virus |
Not Detected |
| Rabies |
Not Detected |
| Reovirus |
Not Detected |
| Cytopathogenic Agents - e.g IBR |
Not Detected |
| Hemadsorbing Agents - e.g. PI3 |
Not Detected |
| Mycoplasma |
|
| Large Volume, Direct Culture |
Not Detected |
| Hoechst DNA Stain |
Not Detected |
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