Viral Vaccine Cell Culture Platform    Product List Product Inserts  - HyClone SFM4MegaVir HyClone SFM4MegaVir Brochure* Application Note: "Production of Poliovirus Using Vero Cells Cultured in HyClone SFM4MegaVir™ with Nunc Cell Factories, T-Flasks, and Roller Bottles" Click Here to Order Hard Copy Versions

The application of serum-free media in the production of virus vaccines has been largely driven by concerns over serum supply and exogenous contaminants. Key cell lines in this industry are the African green monkey kidney cell, Vero, and the canine kidney and bovine kidney epithelial cells, MDCK and MDBK.

HyClone SFM4MegaVir™ (SH30522)
The Premier Protein-Free Medium for Viral Vaccine Production
HyClone SFM4MegaVir is a protein-free medium containing no animal derived components. This regulatory-friendly medium is developed through Metabolic Pathway Design™ to increase process yields in the manufacture of viral vaccines in a variety of cell lines including Vero, COS-7, MDCK, and MDBK.

HyClone SFM4MegaVir has been successfully tested in a variety of culture systems for cell growth and virus production including T-flasks and suspension microcarrier cultures. HyClone SFM4MegaVir does not contain Pluronic^® F68 or L-Glutamine. It does contain phenol red.

Alongside HyClone SFM4MegaVir, HyClone also offers its protein-free medium designed specifically for Vero cells, HyClone PF-Vero™. This medium has been demonstrated superior performance in multiple culture environments, and was the foundation for developing HyClone SFM4MegaVir.

Viral Vaccine Cell Culture Media include:

•	HyClone SFM4MegaVir without L-Glutamine
	> Protein-Free > Animal Derived Component Free > Developed for Multi-Culture Environments, Including Microcarrier Bioreactor Cultures
•	HyClone PF-Vero without L-Glutamine