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Serum-Free Media
HyClone’s versatile serum-free media (SFM) are developed through Metabolic Pathway Design™ to support superior performance in multiple cell culture platforms. This developmental approach produces SFM targeted to increasing process yields for each respective cell platform. Current HyClone SFM applications include hybridoma, NS0, insect, CHO, PER.C6™, HEK 293, MDCK, MDBK, COS-7, and Vero cell cultures. The use of SFM provides many time- and cost-saving advantages, including:

• eliminating the need to pre-screen serum lots
• simplified regulatory documentation
• consistent media performance
• reduced downstream purification challenges

Applications for serum-free media can be found in many biotechnology fields including the production of human and animal biopharmaceuticals, diagnostics reagents and bioagricultural products. Due to the increasing attention to producing biologicals representative of their native form, HyClone’s serum-free media have been focused on supporting mammalian and invertebrate cell culture platforms. Some of the critical cell culture platforms for biotechnology include Chinese Hamster Ovary (CHO) cells, hybridomas and myelomas, insect cells, cells for the production of gene therapy viral vectors, and cells for the production of viral vaccines. These cell culture platforms constitute the majority of applications for biopharmaceutical research, development and manufacture employing eukaryotic cell culture.

Metabolic Pathway Design™
High performance with SFM for specific cell culture platforms is achieved through HyClone’s Metabolic Pathway Design technology. This approach to media formulation development ensures cell productivity during growth and production phases of cell life. HyClone’s extensive work in media development has resulted in products that provide a consistent nutrient supply in a form acceptable to cultured cells.

HyClone’s Metabolic Pathway Design approach balances nutrient supply against metabolic waste accumulation, determines the effective dose of nutrients critical to the production of recombinant proteins, and provides complexed lipids and phospholipids that facilitate delivery through the cell membrane. The objective for developing serum-free media through Metabolic Pathway Design is to provide the intended cell line with the nutrients needed to promote cell growth and stimulate productivity.

PER.C6 is a trademark of Crucell NV