Process Supplement Platform Press Release Product List Product Inserts  - Cell Boost 1  - Cell Boost 2  - Cell Boost 3  - Cell Boost 4  - Cell Boost 5  - Cell Boost 6  - Cell Boost Kit Process Supplements Brochure Click Here to Order Hard Copy Versions

The fed-batch version of stirred-tank culture has become most popular at large scales.The primary driver of this trend is based on adding nutrients to a batch culture in mid-run to increase the quantity of product harvested.The prevalence of fed-batch over other modes is due to many practical factors such as, reliability, ease of scalability and application latitude. In addition, one of the greatest improvements is derived from increases in the integral of viable cell concentration and volumetric productivity.

Primary factors determining optimum feeding of cultures include:

• Preventing toxicity to initial lowdensity seed cultures
• Maintaining desired metabolic pathways in high-density cultures
• Prolonging cell viability and preventing induction of apoptosis
• Promoting longevity of cultures over short-lived peak densities
• Promoting high-quality product accumulation rather than mere quantity
• Encouraging product accumulation rather than simple culture biomass

Today’s experienced fed-batch bioreactor operators can expect more than 10 times the efficiencies possible from a few years ago.Yields, for some secreted recombinant protein biologicals, are now expected to be 1-3 g/L of culture, with 5-10 g/L potentially achievable. As the newest research is applied to production scale, yields exceeding 10 g/L are anticipated.

We have developed a Process Supplements platform to support demand in the area of fed-batch cell culture. Each supplement is developed through the Metabolic Pathway Design™ approach to provide specific nutrient combinations for a variety of applications, and have been successfully tested in CHO, hybridoma, NS0, HEK 293, PER.C6® (Crucell NV) and other cell lines.

The six Cell Boost supplements have been designed to provide such nutrients as amino acids, vitamins, lipids, cholesterol, glucose and/or growth factors in novel combinations for multiple mammalian cell types. HyClone Cell Boost supplements are chemically-defined and contain no animal derived components. In addition to the Cell Boost line, we offers concentrated liquid supplements designed to provide lipids and cholesterol to such cell lines as NS0, as well as specific nutrients for those cells using the glutamine synthetase (GS) Gene Expression System.

Each product in the Process Supplements platform has been rigorously tested in a variety of culture systems, including shaker flask cultures, traditional stirredtank bioreactor cultures and disposable cell culture systems (e.g., theThermo Scientific HyClone Single-Use Bioreactor). Utilizing various serum-free media formulations, the products on the Process Supplements platform have successfully improved cell productivity in some cases more than 10 times what is currently achieved.

Each product in the process supplement platform is Chemically-Defined, Animal Derived Component Free and Protein Free.

HyClone Cell Boost 1™ (R05.2) (SH30584)
HyClone Cell Boost 1 is a supplement designed to provide nutrients such as amino acids, vitamins and glucose. It is designed to improve productivity through a multi-vitamin feed approach. HyClone Cell Boost 1 has been tested on a variety of cell lines including CHO and HEK 293 cells.

HyClone Cell Boost 2™ (R15.4) (SH30596)
HyClone Cell Boost 2 is a supplement designed to provide nutrients such as amino acids and glucose. It is also designed to improve productivity through a single-vitamin feed approach. HyClone Cell Boost 2 has been
tested on a variety of cell lines including CHO and PER.C6®.

HyClone Cell Boost 3™ (JM3.5) (SH30825)
HyClone Cell Boost 3 is a supplement designed to provide nutrients such as glucose, amino acids, vitamins and trace elements. HyClone Cell Boost 3 has been tested on a variety of cell lines including, hybridomas
and myelomas.

HyClone Cell Boost 4™ (R05.2) (SH30857)
HyClone Cell Boost 4 is a supplement designed to provide nutrients such as amino acids, vitamins, glucose, trace elements and growth factors. HyClone Cell Boost 4 has been tested on a variety of cell lines including
CHO.

HyClone Cell Boost 5™ (R15.4) (SH30865)
HyClone Cell Boost 5 is a supplement designed to provide nutrients such as lipids, amino acids, vitamins and growth factors. HyClone Cell Boost 5 has been tested in a variety of cell lines including, CHO, Hybridoma, NS0 and HEK 293.

HyClone Cell Boost 6™ (CN-F) (SH30866)
HyClone Cell Boost 6 is a supplement designed to provide nutrients such as lipids, amino acids, vitamins and growth factors. HyClone Cell Boost 6 has been tested in a variety of cell lines including, CHO, Hybridoma, NS0, HEK 293 and T-cells.

HyClone Cell Boost™ Process Supplement Kit (SH30890)
The HyClone Cell Boost Process Supplements Kit contains 6 x 100 g samples of supplements designed to increase cell productivity in a variety of cell lines. Each supplement is designed to provide nutrients to fed-batch culture applications.The supplements are chemically-defined and contain no animal components.They have been tested in a variety of cell culture systems, including shaker flasks and bioreactors. Each supplement is available in larger packaging configurations.

Process Supplement products available: