Insect Cell Culture Platform    Product List Product Inserts  - HyClone SFX-Insect High Five™ Adaptation to Suspension Culture and SFM GrowthTechnical Brief Production of rAcMNPV Stock Technical Brief Production of rProtein Using BEVS Technical Brief HyClone SFX-Insect Brochure** Click Here to Order Hard Copy Versions

The development of insect cell culture production systems has produced opportunities for the expression of recombinant proteins for research and therapeutic applications in a non-mammalian cell culture environment.

Many different insect cell lines have been developed for use with the baculovirus expression vector system (BEVS). Additionally, the development of stable transfected Drosophila and lepidopteran cell lines has offered a non-lytic culture for the production of recombinant proteins from insect cells.

HyClone SFX-Insect™ (SH30278, SH30350)
The Premier Protein-Free Medium for rProtein Production in Insect Cell Culture
This versatile protein-free cell culture medium is developed through Metabolic Pathway Design™ to support the growth of multiple insect cell lines and production of a variety of recombinant proteins.

It provides superior growth of many key insect cell lines, including Sf9, Sf21, High Five™, and D.mel (2), requiring minimal adaptation. HyClone SFX-Insect is ideally suited for the cost-effective manufacture of recombinant proteins for academic and industrial research, genomics and proteomics, drug target screening and validation, and manufacturing for therapeutic applications.

HyClone SFX-Insect is formulated using HyClone’s proprietary lipid and phospholipid complexing process for enhanced stability and performance.

HyClone SFX-Insect contains Pluronic^® F-68 and has been developed for superior performance in high density bioreactor applications. This protein-free medium has been successfully tested in a variety of culture systems ranging from T-flasks, spinners, shaker flasks (including Fernbach), and various bioreactors including hollow fiber.

In addition to HyClone SFX-Insect, HyClone offers HyClone TNM-FH, HyClone IPL-41, HyClone CCM3™ and the HyClone BEVS PlaKit™. HyClone TNM-FH is a modification of the original Grace’s medium to contain yeast extract and lactalbumin hydrolysate. When supplemented with 10% Insect Screened FBS, TNM-FH becomes a complete insect cell culture medium which requires minimal adaptation. HyClone IPL-41 is a basal medium, frequently used for serum-free media development/optimization. HyClone CCM3 is HyClone’s first serum-free medium for Sf9 cells. The HyClone BEVS PlaKit provides the necessary reagents for a reproducible and accurate baculovirus plaque assay kit.

*High Five is a trademark of Invitrogen Corp.