HEK 293 and PER.C6® Cell Culture    Product List Press Releases  - HEK293  - CDM4PERMAb Product Inserts  - HyClone SFM4HEK293  - HyClone CDM4HEK293  - HyClone CDMPERMAb  - HyClone SFM4Transfx-293 HyClone SFM4HEK293 Direct Adaptation and Cryopreservation Technical Brief HyClone SFM4HEK293 and HyClone CDM4Retino Brochure* Click Here to Order Hard Copy Versions

As biotherapeutics are desired more closely representative of their native form, the application of human-derived cell lines for recombinant protein, monoclonal antibodies and gene therapy viral vectors has rapidly emerged.

Thermo Scientific products include high performing serum-free media capable of providing optimal cell culture conditions for HEK 293 and PER.C6® cells in these applications.These media have been developed through the Metabolic Pathway Design approach to ensure that the needs of human-derived cell lines are met to enable the highest level of productivity.

HyClone CDM4HEK293™ (SH30858, SH30859)
HyClone CDM4HEK293 is a chemically-defined, animal derived component free and protein-free cell culture medium designed to support the growth of HEK 293 cultures, and promote adenovirus and recombinant protein production.This regulatory-friendly medium was developed to support high cell density and specific cell productivity in suspension cultures.

HyClone SFM4Transfx-293™ (SH30860, SH30861)
HyClone SFMTransfx-293 is a serum-free, animal derived component free medium designed to support the growth of HEK 293 cultures and promote transfection using lipofection or similar methods.This regulatory-friendly medium was developed to support high transfection efficiency, productivity and cell density in suspension cultures.

HyClone SFM4HEK293™ (SH30521, SH30522)
The Preferred Protein-Free Medium for Adenoviral Vector and rProtein Production in HEK 293 Cells
This versatile cell culture medium was developed through Metabolic Pathway Design™ to support the growth of HEK 293 cells and the production of adenoviral vectors and rproteins. HyClone SFM4HEK293 is a protein-free medium containing no animal derived components and has been specifically formulated using HyClone’s proprietary lipid complexing process for enhanced stability and broad-spectrum cell culture applications. It contains L-Glutamine and Pluronic^® F-68, and does not contain phenol red.

HyClone SFM4HEK293 has been successfully tested in a variety of culture systems including T- flasks, shakers, roller bottles, and bioreactors, including perfusion.

HyClone CDM4PERMAb™ (SH30871, SH30872)
HyClone CDM4PERMAb is a chemically-defined medium containing no animal derived components.This high performance cell culture medium has been developed to increase process yields in the production of human antibodies and recombinant proteins using PER.C6® technology. It has been successfully tested in a variety of applications, including fed-batch bioreactors.

HyClone CDM4Retino™ (SH30520, SH30519)
The Premier Protein-Free Medium for Adenoviral Vector And rProtein Production in PER.C6 Cells
This high performance cell culture medium is developed through Metabolic Pathway Design™ to increase the process yields for the industrial manufacture of adenoviral vectors and rproteins using PER.C6 and other retinoblast cells.

HyClone CDM4Retino is a chemically-defined medium containing no proteins, animal derived components, hydrolysates or other undefi ned components. It is a regulatory-friendly medium capable of producing superior cell yields in a variety of culture environments and applications, including perfusion bioreactors.

HyClone CDM4Retino is formulated using a proprietary lipid complexing process for enhanced stability and growth promotion of various cell types.

In addition to these new gene therapy products, HyClone is pleased to continue offering HyClone PF-293™, our first generation protein-free medium for HEK 293 cells.

Products available for HEK 293 and PER.C6 cells include:

•	HyClone SFM4HEK293 with L-Glutamine
	> Protein-Free, ADCF > Designed for High Performance Suspension HEK293 Cultures
•	HyClone CDM4Retino with L-Glutamine
	> Chemically Defined, ADCF > Designed for High Performance Suspension PER.C6 Cultures
•	HyClone PF-293

PER.C6 is a trademark of Crucell NV