Dissociation products are used to disaggregate adherent cells from the surface or substrate to which they are attached. HyClone offers both gamma irradiated porcine trypsin products and a new non-mammalian trypsin replacement called HyQ®Tase™.

HyQTase cell detachment solution is a naturally derived complex of proteolytic and collagenolytic enzymes. It does not contain mammalian or bacterial derived products and is non-recombinant. HyQTase enables rapid cell detachment while being gentle to cells and does not require inhibition. It is ideal for use in both serum-free and serum-containing cell culture systems with a broad array of cell lines. It is effective for tissue dissociation for primary cell culture and for routine detachment of adherent cells from various substrates, including standard tissue culture plasticware and adhesion coated plasticware. Formulation includes 1X HyQTase enzymes in a solution of phosphate buffered saline with 0.5mM EDTA. HyQTase should be stored frozen and is stable for up to 60 days at 4º C.

Trypsin is derived from porcine pancreas and is gamma irradiated prior to hydration and filling. HyClone offers three formulations as described below. All are without calcium and magnesium and should be stored frozen (-5 to -20ºC).

• 0.25% (2.5 g of 1:250/L Porcine Trypsin) in HBSS with 0.2 g/L EDTA
• 0.05% (0.5 g of 1:250/L Porcine Trypsin) in HBSS with 0.2 g/L EDTA
• 0.25% (2.5 g of 1:250/L Porcine Trypsin) in HBSS with 1.0 g/L EDTA