Hygromycin B is an aminoglycosidic antibiotic that kills bacteria, fungi and higher eukaryotic cells by inhibiting protein synthesis. The resistance gene codes for a kinase that inactivates Hygromycin B through phosphorylation. Cloning of the resistance gene and fusion with eukaryotic promoters has resulted in the development of vectors that permit selection for resistance to Hygromycin B in both prokaryotic and eukaryotic cells. it is useful as an antifungal or antibacterial. Hygromycin B inhibits protein synthesis by disrupting translocation and promoting mistranslation of the 80S ribosome. It is used for the selection and maintenance of prokaryotic and eukaryotic cells transfected with the hygromycin resistance gene, hph.

Working concentration for mammalian cell seletion is normally between 50 µ/mL and 1 mg/mL; for plant cells: 20-200 µg/mL, for bacteria: 20-200 µg/m, and for fungi: 200 µg-1 mg/mL.

Description			Part Number	Size	Stock/MTO	Storage Temp
Hygromycin B	Solution, 50 mg/mL		SV30070.01	20 mL	Stock	2-8°C