|
Providing optimal culture conditions can be one of the greatest challenges
in stem cell research, particularly in keeping cells in an undifferentiated
state or directing differentiation when desired.
The Thermo Scientific HyClone AdvanceSTEM line of ES-Qualified cell culture
products can help you meet those challenges.
Ordering Information:
| Description |
Part
Number |
Packaging |
AdvanceSTEM™
|
IMDM4SC |
SH30822.01 |
500
mL |
AdvanceSTEM™
|
DMEM4SC without L-Glutamine |
SH30824.01 |
500
mL |
AdvanceSTEM™
|
ES
Qualified HEPES |
SH30851.01 |
100
mL |
AdvanceSTEM™
|
ES Qualified L-Glutamine
|
SH30852.01 |
100
mL |
AdvanceSTEM™
|
ES Qualified NEAA
|
SH30853.01 |
100
mL |
Table 1
| Brand |
Amount
for 250 mL |
Product |
Catalog
Number |
| Thermo
Scientific |
205
mL |
HyClone
AdvanceSTEM™ DMEM4SC |
SH30824 |
| Thermo
Scientific |
35
mL |
HyClone
ES Screened FBS |
SH30070(E) |
| Thermo
Scientific |
2.5
mL |
HyClone
AdvanceSTEM™ ES Qualified
L-glutamine 200mM |
SH30852 |
| Thermo
Scientific |
2.5
mL |
HyClone
AdvanceSTEM™
ES Qualified Non-Essential
Amino Acids (NEAA) 100X |
SH30853 |
| Thermo
Scientific |
2.5
mL |
Penicillin/Streptomycin
Solution (optional) |
SV30010 |
| Thermo
Scientific |
2.5
mL |
HyClone
AdvanceSTEM™
ES Qualified HEPES (1M) |
SH30851 |
| Fisher |
2.5
ul |
2-ME |
ICN19470580 |
| Millipore |
25
ul |
ESGRO
LIF |
ESG1107 |
| Mix
all ingredients in the top of 250 mL PES filter 0.22 µm unit
and filter sterilize. Store at 4 C. Discard unused medium after 10
days. |
Pluripotent B6-ESC's have a very distinct morphology when cultured under
certain conditions; growing as tightly-clustered colonies with smooth
phase bright borders. The PRX- B6 ESC's grow quickly and require daily
maintenance. When co-cultured in the medium described in Table 2 with
mouse embryonic fibroblasts (MEFs) these B6 ESC are very robust.
Table 2
| Brand |
Amount
for 200 mL |
Product |
Catalog
Number |
| Thermo
Scientific |
145
mL |
HyClone
AdvanceSTEM™ IMDM4SC |
SH30822 |
| Thermo
Scientific |
40
mL |
HyClone
ES Screened FBS |
SH30070(E) |
| Thermo
Scientific |
2.0
mL |
HyClone
AdvanceSTEM™ ES Qualified
L-glutamine 200mM |
SH30852 |
| Thermo
Scientific |
2.0
mL |
HyClone
AdvanceSTEM™
ES QualifiedNon-Essential Amino
Acids (NEAA) 100X |
SH30853 |
| Thermo
Scientific |
2.0
mL |
Penicillin/Streptomycin
Solution (optional) |
SV30010 |
| Thermo
Scientific |
2.0
mL |
HyClone
AdvanceSTEM™
ES Qualified HEPES (1M) |
SH30851 |
| Fisher |
3.0
ul |
2-ME |
ICN19470580 |
| Millipore |
20
ul |
ESGRO
LIF |
ESG1107 |
| Mix
all ingredients in the top of 250 mL PES filter 0.22 µm unit
and filter sterilize. Store at 4 C. Discard unused medium after 10
days. |
|
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